|
|
TABLE 2.
Preliminary microbiological characterization of
subsamples from a black smoker chimney at the PACMANUS
sitea
|
|
| Subsample no. |
Total
amt (g) |
Cell density (cells/g [wet wt]) |
DNA yield (ng/g
[wet wt]) |
RNA yield (ng/g [wet wt]) |
rRNA/rDNA
composition (%) for:
|
| Bacteria |
Archaea
|
|
| I |
30 |
1.0 × 106 |
5.1 |
2.2 |
78.3/92.6 |
19.9/7.4
|
| II |
15 |
3.2 × 107 |
169 |
100 |
66.8/94.7 |
30.5/5.3
|
| III |
36 |
4.5 × 105 |
3.2 |
1.3 |
97.6/100 |
1.8/ND
|
| IV |
18 |
4.5 × 105 |
1.6 |
1.4 |
98.5/97.2 |
2.8/2.8
|
| V |
19.5 |
8.0 × 105 |
3.0 |
4.5 |
95.1/100 |
2.2/ND
|
| VI |
15 |
5.1 × 105 |
1.6 |
1.7 |
76.7/92.8 |
23.1/7.2 |
|
|
a
Prokaryotic universal, bacterial, and
archaeal SSU rRNA/rDNA composition was quantified by using RNA dot
hybridization or quantitative fluorogenic PCR (52). The
proportions represent the result by RNA dot hybridization divided by
the result by quantitative fluorogenic PCR. ND, not detected.
|