TABLE 4.   Viable-cell counts of Thermococcus members and halophilic microorganisms calculated by three-tube MPN cultivation methoda


Subsample no. Viable-cell count in:
MJYPS medium for Thermococcus (cells g [wet wt]-1)
HA medium for Haloarcula (cells g [wet wt]-1)
55°C 75°C 90°C 30°C 45°C

I 4.6 × 102 2.4 × 102 3.9 × 101 ND ND
II 2.1 × 104 1.5 × 104 9.0 × 103 ND ND
III 7.5 × 102 4.3 × 102 7.5 × 101 ND ND
IV 9.0 × 100 9.0 × 100 ND 9.3 × 101 ND
V ND ND ND 9.3 × 102 9.3 × 101
VI ND ND ND 7.5 × 102 9.0 × 101

a Viable-cell counts obtained by the MPN method using HA medium represent not Haloarcula but halophilic bacteria such as Halomonas spp, as described in Results. ND, not detected.