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Appl Environ Microbiol. 1962 January; 10(1): 90-92

A Transparent Solid Medium for Growth Enhancement of Pasteurella tularensis¹

Department of Microbiology, University of Maryland, College Park, Maryland

ABSTRACT

A transparent plating medium capable of initiating early and rapid growth of the SCHU S4 strain of Pasteurella tularensis is described. On this medium, colonies 1 mm in diameter were obtained following 29 hr of incubation at high humidity. To obtain this growth enhancement, blood factors were provided in the form of a peptic digest of hemoglobin and a saline wash of red blood cells (rabbit). In the basal medium, the presence of both 0.5% L-cysteine-HCl, and sodium thioglycolate, was required to achieve this growth response.

² Professor and Head of the Department of Microbiology, University of Maryland.

¹ Taken in part from a thesis submitted by the senior author to the Faculty of the Graduate School of the University of Maryland in partial fulfillment of the requirements for the M.S. degree, June, 1961.