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Appl Environ Microbiol. 1962 September; 10(5): 463-465

Attempts to Freeze Some Bacteriophages to Ultralow Temperatures

William A. Clark, Wanda Horneland and A. G. Klein

American Type Culture Collection, Washington, D.C.

ABSTRACT

A variety of bacteriophages specific for different hosts, including Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Shigella dysenteriae, Staphylococcus aureus, and Vibrio comma, were frozen at a controlled rate to liquid nitrogen temperatures, and then quick-thawed. Glycerol (10%) was used as a protective additive. Quantitative determinations showed from 10% to virtually 100% recovery in the various cases. Host specificity, plaque morphology, and, in one case, rate of inactivation of phage by homologous antiserum remained unchanged.

Appl Environ Microbiol. 1962 September; 10(5): 463-465






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