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Appl Environ Microbiol. 1963 March; 11(2): 157-162

Multicomponent Substrate Utilization by Natural Populations and a Pure Culture of Escherichia coli

Division of Bio-engineering, School of Civil Engineering, Stillwater, Oklahoma
Microbiology Department, Oklahoma State University, Stillwater, Oklahoma

ABSTRACT

A heterogeneous population, typical of activated sludge, and a prototrophic strain of Escherichia coli were used to test for sequential substrate removal in a glucose-sorbitol medium. Each culture was preacclimated to sorbitol and was studied in the two-component medium under growing and nonproliferating conditions. In all four systems, glucose blocked sorbitol removal. Since large initial inocula were used, the suppression of sorbitol metabolism could not be totally due to repression of enzyme synthesis. The results indicate that glucose may affect the functioning of an existing enzyme system in addition to its established effect on enzyme synthesis. From an applied standpoint, the results indicate that an activated sludge may be completely and immediately prevented from eliminating a waste constituent to which it is acclimated.