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Appl Environ Microbiol. 1963 July; 11(4): 320-325

Fractionation of ß-Glucosidases and Related Extracellular Enzymes from Aspergillus niger

L.-h. Li and K. W. King

Department of Biochemistry and Nutrition, Virginia Polytechnic Institute, Blacksburg, Virginia

ABSTRACT

Industrial concentrates from Aspergillus niger culture filtrates were fractionated by ion-exchange and adsorption chromatography. Several other types of hydrolases were completely removed. Eight partially purified components were obtained. Using specific activity as an estimate of purification, one aryl-ß-glucosidase was purified 35-fold. Another component showed 147-fold purification using a viscosimetric assay with carboxymethylcellulose as substrate. The aryl-ß-glucosidase was distinctly more thermolabile than the carboxymethylcellulase.

Appl Environ Microbiol. 1963 July; 11(4): 320-325






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