This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Pepper, R. E. Articles by Chandler, V. L. Search for Related Content PubMed Articles by Pepper, R. E. Articles by Chandler, V. L. Agricola Articles by Pepper, R. E. Articles by Chandler, V. L.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1963 September; 11(5): 384-388

Sporicidal Activity of Alkaline Alcoholic Saturated Dialdehyde Solutions

Department of Microbiology, Ethicon, Inc., Somerville, New Jersey

ABSTRACT

During a search for a sporicidal agent to equal or surpass formaldehyde in activity, a group of saturated dialdehydes ranging from two to six carbons was discovered. These dialdehydes exerted a surprisingly high degree of activity in isopropanol buffered with a carbonate or bicarbonate. Without the proper buffer, practically no activity was observed. A solution of 1% glutaraldehyde, 0.3% NaHCO₃, and 70% isopropanol sterilized stainless-steel penicylinders contaminated with standardized numbers of four spore types in a shorter time than did commercially available 8% formaldehyde solution. Both glyoxal and glutaraldehyde in isopropanol destroyed spores in a relatively short time. Care was taken to eliminate bacteriostasis. Various tests were performed to evaluate accurately experimental and formaldehyde solutions.

¹ Present address: Department of Microbiology and Public Health, Michigan State University, East Lansing.

² Deceased.