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Appl Environ Microbiol. 1963 September; 11(5): 453-457

Effect of Carbohydrates in Phosphate Buffer on Germination of Bacillus stearothermophilus Spores¹

Food Microbiology Research Laboratories, Department of Horticulture, University of Missouri, Columbia, Missouri

ABSTRACT

Among strains, and among spore suspensions of the same strain, different spore-germination responses were observed when spores were heated in monosaccharides, disaccharides, and polysaccharides in 0.0083 M phosphate buffer (pH 7.1). It was hypothesized that these differences were due to rough and smooth variants in the spore population and to variation in the osmosensitivity of spores of variants within the population when subjected to a heat shock of 110 C.

¹ Contribution from the Missouri Agricultural Experiment Station; journal series no. 2601 as approved by the Director.