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Appl Environ Microbiol. 1963 November; 11(6): 463-466

Production of Pectolytic Enzymes by Rhizopus stolonifer Sporangiospores After "Lethal" Gamma Irradiation

N. F. Sommer, Min T. Creasy, E. C. Maxie and R. J. Romani

Department of Pomology, University of California, Davis, California

ABSTRACT

Irradiated sporangiospores of Rhizopus stolonifer excreted pectolytic enzymes, which hydrolyzed pectin and macerated potato tuber discs, into the suspending medium. Pectin glycosidase, but not pectin methylesterase, activity developed regardless of the amount of radiation the spores had received, unless the dose exceeded about 1 megarad. The ability to produce pectolytic enzymes was found to be more radiation-resistant than the potential for colony formation or the ability to germinate. Spores made incapable, through irradiation, of forming colonies continued to produce pectolytic enzymes after a 6-day period following irradiation treatment.


Appl Environ Microbiol. 1963 November; 11(6): 463-466







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