This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gallup, D. M. Articles by Gerhardt, P. Search for Related Content PubMed Articles by Gallup, D. M. Articles by Gerhardt, P. Agricola Articles by Gallup, D. M. Articles by Gerhardt, P.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1963 November; 11(6): 506-512

Dialysis Fermentor Systems for Concentrated Culture of Microorganisms

Department of Microbiology, The University of Michigan, Ann Arbor, Michigan

ABSTRACT

Cell-mass production of Serratia marcescens was studied in dialysis systems in which growth was managed in a fermentor remote from, but connected by conduits and pumps with, a nutrient reservoir. Dialysis was accomplished with membrane tubing in either the fermentor or the reservoir, or best with membrane sheet in a plate-and-frame dialyzer that was remote from but connected with both vessels. Growth trials with these systems demonstrated their ability to produce virtually unlimited population density in a liquid culture, viable counts in excess of 10¹² cells/ml and partial cell volume of 50% being attained. The system used for growth also may be used, after the growth cycle, to concentrate cells still further by osmotic dehydration with a hydrophilic colloid. The dialyzer-dialysis system that was evolved permits independent control of the component operations and is believed to be adaptable to any desired scale of size.