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Appl Environ Microbiol. 1963 November; 11(6): 523-528

Microbiological Production of Gibberellic Acid in Glucose Media¹

Escuela Nacional de Ciencias Quimícas, Universidad de México, Mexico, D.F., Mexico

ABSTRACT

Gibberellic acid production from various substrates was studied in 43 strains of Fusarium, among which F. moniliforme strain IOC-3326 was selected as the best producer. Experiments were carried out in shaker flasks and pilot plant fermentors. The results indicate that the best substrate for gibberellic acid production with this strain is composed of the following: glucose, 20 g; corn steep liquor, 25 g; ammonium nitrate, 2.6 g; monopotassium phosphate, 0.5 g; potassium sulfate, 0.2 g; and water, 1000 ml. Glucose, ammonium nitrate, and corn steep liquor were found to be critical. With this medium, maximal yields of 1196 mg per liter in shaker flasks and 997 mg per liter in fermentors were produced.

¹ This paper was presented at the II Latin-American Congress of Microbiology, San José, Costa Rica, 10-16 December 1961.