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Appl Environ Microbiol. 1964 January; 12(1): 46-52
Copyright © 1964 by the American Society for Microbiology. All Rights Reserved.

Production of Hydrogen Sulfide by Streptomycetes and Methods for its Detection

Department of Industrial Microbiology, University College, Dublin, Ireland

ABSTRACT

The ability of streptomycetes to produce hydrogen sulfide is generally used for taxonomic purposes. It was found that the previously used method, the blackening of Peptone Iron Agar, does not clearly indicate formation of hydrogen sulfide. It was shown that the blackening of a lead acetate strip is the most accurate indicator for H₂S-producing streptomycetes. A great variety of organic and inorganic sulfur compounds were examined and compared, and the choice of the most suitable sulfur source and method for the detection of hydrogen sulfide is discussed.

¹ Present address: Hartley Botanical Laboratories, University of Liverpool, Liverpool, England.