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Appl Environ Microbiol. 1964 May; 12(3): 188-194
Copyright © 1964 American Society for Microbiology. All Rights Reserved.

Survival of Shigella in Sewage

II. Effect of Glycerol on Shigella flexneri and Shigella Bacteriophage¹

Veterans Administration Hospital and Department of Microbiology, University of Colorado School of Medicine, Denver, Colorado

ABSTRACT

Glycerol (30%) inhibited or delayed the adsorption of Shigella bacteriophage on its host organism, S. flexneri II; glycerol also inhibited or delayed the burst of phage, whether or not adsorption was carried out in the presence of glycerol. Studies of the mechanisms of these effects showed that viscosity and osmotic shock probably were not responsible for either phenomenon. The inhibition of adsorption, however, was proportional to the concentration of glycerol, and appeared to be a function of the hydroxyl groups on the glycerol molecule. The inhibition of burst seemed to be related to the osmotic pressure outside the bacterial cells.

¹ Presented in part at the Annual Meeting of the American Society for Microbiology, Kansas City, Mo., 6-10 May, 1962.