AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thiemann, J. E.
Right arrow Articles by Licciardello, G.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Thiemann, J. E.
Right arrow Articles by Licciardello, G.
Agricola
Right arrow Articles by Thiemann, J. E.
Right arrow Articles by Licciardello, G.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1964 May; 12(3): 261-268
Copyright © 1964 American Society for Microbiology. All Rights Reserved.

Rifamycin1

XXXIII. Isolation of Actinophages Active on Streptomyces mediterranei and Characteristics of Phage-Resistant Strains

J. E. Thiemann, C. Hengeller, A. Virgilio, O. Buelli and G. Licciardello

Lepetit S.p.A., Research Laboratories, Milan, Antibiotici Lepetit, Naples, Italy

ABSTRACT

Five actinophages highly specific for Streptomyces mediterranei were isolated from lysed broth cultures. Studies were performed on the effect of plating conditions on plaque formation. The development of phage-resistant strains of S. mediterranei not only eliminated the phage but also significantly increased rifamycin yields. The phage-resistant cultures proved to be more unstable than the original sensitive strain. Maintenance of the cultures as frozen vegetative mycelium assured culture stability and reproducibility of the results. Strict aseptic precautions throughout the laboratories and fermentation areas did not eliminate the danger of phage infection; effective control was obtained only with the introduction of resistant strains. S. mediterranei phages proved to be highly specific for calcium as an adsorption cofactor; addition of calcium-sequestering agents to sensitive mycelium completely prevented its lysis by the phage. The resistant strains developed were capable of adsorbing the phage and of releasing it without multiplication upon aging of the mycelium. No marked morphological, cultural, or biochemical differences were found among the various phage-resistant strains.

FOOTNOTES

1 The name "rifamycin" has been adopted instead of "rifomycin" as in previous papers to avoid confusion with other antibiotics.

Appl Environ Microbiol. 1964 May; 12(3): 261-268
Copyright © 1964 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1964 by the American Society for Microbiology. All rights reserved.