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Appl Environ Microbiol. 1964 July; 12(4): 289-291
Copyright © 1964 American Society for Microbiology. All Rights Reserved.

Utilization of a Stable Isotope of Oxygen by Pseudomonas fluorescens

Bioengineering Laboratory, Rose Polytechnic Institute, Terre Haute, Indiana

ABSTRACT

Oxygen utilization is defined in this investigation as the terminal use of oxygen in respiration, i.e., the formation of water. A culture of Pseudomonas fluorescens was allowed to respire in an atmosphere of O¹⁸. The production of H₂O¹⁸ was measured during two test runs of 124 and 232 min each. During the first run, 0.505 mmole of H₂O¹⁸ was produced. The second run produced 0.460 mmole of H₂O¹⁸. H₂O¹⁸ production took place throughout the course of each of the runs.