This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Meyer, R. C. Articles by Kohler, E. M. Search for Related Content PubMed Articles by Meyer, R. C. Articles by Kohler, E. M. Agricola Articles by Meyer, R. C. Articles by Kohler, E. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1964 July; 12(4): 295-300
Copyright © 1964 American Society for Microbiology. All Rights Reserved.

Procurement and Maintenance of Germ-Free Swine for Microbiological Investigations¹

Department of Microbiology, The Ohio State University, Columbus, Ohio

ABSTRACT

Germ-free swine were routinely procured by both hysterectomy and hysterotomy (Caesarian section). By means of light-weight portable equipment, piglets could be obtained and transported to the laboratory (without contamination) over distances in excess of 100 miles. The isolators employed in rearing were constructed of stainless steel and flexible plastic film. At weekly intervals, fecal swabs and waste from the floor of the isolator were cultured on blood-agar and in thioglycolate broth, as well as being examined microscopically for the presence of bacteria, yeast, and fungi. The presence of pleuropneumonia-like organisms (PPLO) and viruses in such material was not demonstrable, either by the use of enriched PPLO media or primary porcine-kidney cell cultures. Tissues, body fluids, and cecal contents of piglets sacrificed specifically for microbiological examination were also negative for PPLO, viruses, bacteria, yeast, and fungi. Prenatal infestations by ascarids were not observed. Nutritional problems related to rearing of germ-free piglets, such as hypoglycemia, were not encountered, and the use of an autoclaved commercial sow's milk replacer proved quite satisfactory. The temperature to which piglets were subjected during the first few days of life, however, was very important. The isolator design and application of gnotobiotic techniques to the procurement and rearing of a large germ-free animal such as the pig proved feasible and less difficult than anticipated.

² Present address: National Cancer Institute, National Institutes of Health, Bethesda, Md.

¹ Presented at the 63rd Annual Meeting of the American Society for Microbiology, Cleveland, Ohio, 5-9 May 1963.