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Appl Environ Microbiol. 1964 July; 12(4): 355-359
Copyright © 1964 American Society for Microbiology. All Rights Reserved.

Improved Staphylococcus Medium No. 110

Department of Bacteriology, University of Southern California, Los Angeles, California

ABSTRACT

Most media have been unreliable when used to determine the number of coagulase-positive staphylococci in meat pot pies in the presence of overwhelming numbers of Bacillus cells. Various metabolic inhibitors were tested to determine whether they would suppress the growth of Bacillus cells without appreciably affecting the staphylococcal growth. The use of Staphylococcus Medium No. 110 modified by the addition of 0.75 mM sodium azide appears to be practical for the isolation of small numbers of coagulase-positive staphylococci from frozen meat pot pies.