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Appl Environ Microbiol. 1964 November; 12(6): 475-478
Copyright © 1964 American Society for Microbiology. All Rights Reserved.

Milk-Clotting Enzymes From Microorganisms

National Dairy Research Institute, Karnal, India

ABSTRACT

A total of 230 cultures of fungi and 43 cultures of bacteria, isolated from such sources as soil, butter, and milk, were screened for their milk-clotting activity. The fungi were cultivated on semisolid media, and the bacteria were grown in milk media in shake culture. Phytic acid, added as calcium phytate, was found to stimulate production of the enzyme in most of the bacterial isolates. Proteolytic activity was invariably found to be associated with the milk-clotting enzyme in bacterial isolates. There was considerable variation in the ratio of the two enzymes from strain to strain.