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Appl Environ Microbiol. 1965 January; 13(1): 115-119
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Effect of Oxygen-Supply Rates on Growth of Escherichia coli

II. Comparison of Results in Shake Flasks and 50-Liter Fermentor

Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey

ABSTRACT

Growth of Escherichia coli and chemical changes in the medium were very similar in highly baffled flasks and in a 50-liter fermentor run under the same oxygen-supply conditions, based on sulfite-oxidation rates. Flasks with stainless-steel baffles (Biotech) gave growth patterns and rates of glucose and NH₄-N utilization almost identical to those of the fermentor; results with Bellco 598 flasks (with 6 to 7 mm deep indentations) were quite similar. Unbaffled and Bellco 600 flasks (3 to 4 mm indentations) were similar to the fermentor at very high and very low oxygen-transfer rates, but gave much less growth than the fermentor at intermediate levels. Maximal oxygen-uptake rates occurred in the fermentor at the end of the logarithmic-growth phase when growth was 40 to 75% of maximum. In the fermentor, both sulfite-oxidation rates and rates of oxygen uptake correlated reasonably well with the total amount of growth produced.