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Appl Environ Microbiol. 1965 January; 13(1): 81-85
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Application of a Microtechnique to the Agglutination Test for Leptospiral Antibodies1

Mildred M. Galton, Catherine R. Sulzer, C. A. Santa Rosa and Michael J. Fields

Veterinary Public Health Laboratory, Communicable Disease Center, Epidemiology Branch, U.S. Public Health Service, Atlanta, Georgia
Instituto Biologico, Sao Paulo, Brazil

ABSTRACT

A microtechnique has been developed and adapted successfully to the microscopic agglutination test with live antigens for detection of leptospiral antibodies. Simultaneous titrations were performed by the conventional microscopic agglutination test and the microtechnique. When the microtechnique was used to screen 50 unknown leptospiral strains with a battery of hyperimmune sera, 98% agreement was obtained with the conventional procedure. Comparative data on 635 tests on these 50 cultures established the reliability of the microtechnique. Results with the two tests on 46 human sera revealed 93% agreement in the detection of leptospiral antibodies. The validity and reliability of the microtechnique obtained in these comparative studies suggests that it can be used as a valuable screening procedure for the microscopic agglutination test for preliminary cross agglutination studies on unknown strains and for the detection of leptospiral antibodies in human and animal sera.

FOOTNOTES

1 Presented at the 64the Annual Meeting of the American Society for Microbiology, 4 May 1964, Washington, D.C.

Appl Environ Microbiol. 1965 January; 13(1): 81-85
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





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