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Appl Environ Microbiol. 1965 January; 13(1): 93-98
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Chemostatic Concentrated Cultures of Heteroploid Mammalian Cell Suspensions in Dialyzing Fermentors

I. Experimental Evidence and Theoretical Considerations

Gio B. Gori

Microbiological Associates, Inc., Bethesda, Maryland

ABSTRACT

Concentrated chemostatic cultures of HeLa S3-1, KB, and HEp # 2 cells have been grown in a dialysis fermentor. Stationary cell concentrations of approximately 1.2 x 106 cells per ml have been produced at rates of 15 x 10-3 to 20 x 10-3 cells per hour for as long as 40 days. The dialysis fermentor appears to be useful in controlling the effects of nutrients on the growth rate of the cultures. Theoretical considerations are offered.

Appl Environ Microbiol. 1965 January; 13(1): 93-98
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





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Copyright © 1965 by the American Society for Microbiology. All rights reserved.