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Appl Environ Microbiol. 1965 January; 13(1): 99-102
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

New Method for Purifying Coxiella burnetii by Dextran Sulfate Precipitation Followed by Centrifugation

J. L. Davis and W. C. Patrick Iii

U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland

ABSTRACT

This paper describes a simple method for purifying Coxiella burnetii cultivated in the chick embryo. The procedure is based upon the preliminary clarification of infected whole-egg suspensions by precipitating the low-density lipoproteins and other extraneous solids with dextran sulfate, calcium chloride, and sodium chloride solutions, and then concentrating the rickettsiae by centrifugation.

Appl Environ Microbiol. 1965 January; 13(1): 99-102
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





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Copyright © 1965 by the American Society for Microbiology. All rights reserved.