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Appl Environ Microbiol. 1965 March; 13(2): 175-180
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Absorption and Fluorescence of Water-Soluble Pigments Produced by Four Species of Pseudomonas

Eastern Regional Research Laboratory, U.S. Department of Agriculture, Philadelphia, Pennsylvania

ABSTRACT

Pigments produced by four species of Pseudomonas during growth in three media were examined for visible and ultraviolet absorption. Ultraviolet fluorescence excitation and emission spectra were obtained. In all pigments, an absorption maximum occurs at 405 mµ. Ultraviolet excitation of fluorescence occurs primarily at 400 to 410 mµ, with smaller maxima at 460 mµ, or 525 mµ, depending on pH, bacterial species, or medium. Emission maxima, after excitation at 410 mµ, occur at 390 mµ, and 455 to 475 mµ. The differences in fluorescence spectra may be used for taxonomic classification of the Pseudomonas.