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Appl Environ Microbiol. 1965 March; 13(2): 208-211
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Production of Aflatoxins in Submerged Culture¹

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts

ABSTRACT

Aflatoxins can be produced on a synthetic medium in submerged culture. Glucose, sucrose, or fructose are the preferred carbon sources, and Casamino Acids are the preferred nitrogen source. Ammonia is almost as good a nitrogen source. Zinc is required at levels of at least 0.4 mg per liter. Concentrations of aflatoxin of 60 to 80 mg per liter (as determined by optical-density measurements of a chloroform extract of the unfiltered broth) can readily be obtained in indented shake flasks; somewhat lower yields were obtained in 5-liter fermentors.

¹ Presented in part at the 148th meeting of the American Chemical Society, Chicago, September, 1964. Contribution no. 632 from the Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge.