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Appl Environ Microbiol. 1965 March; 13(2): 254-256
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Cooling Device for Use with a Sonic Oscillator

Theodore Rosett

Department of Biochemistry, Duke University Medical Center, Durham, North Carolina

ABSTRACT

A cooling cell is described that facilitates maintenance of biological materials at low temperatures during prolonged sonic treatment. Using this cell and a Branson S-75 Sonifier, I examined temperatures and the release of protein and enzyme activity from suspensions of Saccharomyces cerevisiae. With the Sonifier at full power, it was possible to maintain cell temperature within 9 C of the cooling-bath temperature, and to disrupt 10% (w/v) suspensions of S. cerevisiae in 10 min.

Appl Environ Microbiol. 1965 March; 13(2): 254-256
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





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Copyright © 1965 by the American Society for Microbiology. All rights reserved.