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Appl Environ Microbiol. 1965 July; 13(4): 559-563
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Rapid Technique for the Enumeration of Clostridium perfringens

Department of Bacteriology, Indiana University, Bloomington, Indiana

ABSTRACT

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.

¹ Present address: Division of Natural Sciences, State University of New York at Brockport, Brockport, N.Y.