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Appl Environ Microbiol. 1965 July; 13(4): 575-578
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Characterization of Certain Gram-Negative Bacteria from Surface Waters¹

Department of Civil Engineering and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin

ABSTRACT

Cultures of gram-negative bacteria with oxidative glucose metabolism were isolated from surface waters by a highly selective technique, and were classified into 11 types. The predominant type, making up about 50% of the isolated cultures, was cytochrome oxidase-positive, produced fluorescent pigment, and failed to grow at 37 C. A similar type, which differed in being cytochrome oxidase-negative, constituted about 10% of the isolates. Both types of bacteria probably were composed of more than one species. A third type, composed of purple-pigmented pseudomonads, made up approximately 30% of the isolated cultures, and probably represented the predominant species. Other bacterial types isolated constituted less than 10% of the cultures examined.

¹ Engineering Experiment Station reprint no. 756.