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Appl Environ Microbiol. 1965 September; 13(5): 680-685
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Use of the Stereoisomers of Isoleucine by Lactobacillus fermenti

Osaka Research Laboratory, Tanabe Seiyaku Company, Ltd., Osaka, Japan

ABSTRACT

The use of four stereoisomers of isoleucine by Lactobacillus fermenti strain 36 was studied in detail. All four isoleucine isomers were used for growth in the presence of vitamin B₆ compounds, but only L-isoleucine was active in the absence of these vitamins. Of the vitamin B₆ compounds, pyridoxal and pyridoxamine were equally more effective than pyridoxine for the utilization of these isomers. Lowering the initial pH, decreasing the amounts of leucine and valine, and adapting the organism to D-alloisoleucine medium accelerated the use of isoleucine isomers. Thus, the conditions were established under which respective isomers gave the same growth response, and these findings were applied to the separate microbiological assay of L-isoleucine and total isoleucine isomers.