This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Payne, W. J. Articles by Mayberry, W. R. Search for Related Content PubMed Articles by Payne, W. J. Articles by Mayberry, W. R. Agricola Articles by Payne, W. J. Articles by Mayberry, W. R.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1965 September; 13(5): 698-701
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Primary Alcohol Sulfatase in a Pseudomonas Species

Department of Bacteriology, University of Georgia, Athens, Georgia

ABSTRACT

An ammonium sulfate-precipitated fraction from cell-free extracts of Pseudomonas C12B grown on a medium containing sodium dodecyl sulfate (SDS) contained alkyl sulfatase increased fourfold in specific activity over the crude. Optimal pH (7.5) and temperature (70 C) for sulfate release were determined with SDS labeled with radioactive sulfur (SDS³⁵) as test substrate. Phosphate, arsenate, and certain heavy metal ions inhibited desulfation, whereas Mg⁺⁺ and Mn⁺⁺ stimulated activity of preparations which had been dialyzed against ethylenediaminetetraacetic acid. Dodecanol was recovered in semiquantitative yield from reaction mixtures containing enzyme and SDS³⁵. Aryl sulfates, secondary alcohol sulfates, and a phenoxyethyl sulfate failed to serve as substrate for this enzyme.