AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Corlett, D. A.
Right arrow Articles by Sinnhuber, R. O.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Corlett, D. A., Jr.
Right arrow Articles by Sinnhuber, R. O.
Agricola
Right arrow Articles by Corlett, D. A.
Right arrow Articles by Sinnhuber, R. O.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1965 September; 13(5): 808-817
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Application of Replica Plating and Computer Analysis for Rapid Identification of Bacteria in Some Foods1

I. Identification Scheme

D. A. Corlett Jr.2, J. S. Lee and R. O. Sinnhuber

Department of Food Science and Technology, Oregon State University, Corvallis, Oregon

ABSTRACT

A method was devised and tested for a quantitative identification of microbial flora in foods. The colonies developing on the initial isolation plates were picked with sterile toothpicks and inoculated on a master plate in prearranged spacing and order. The growth on the master plates was then replicated on a series of solid-agar plates containing differential or selective agents. The characteristic growth and physiological responses of microbial isolates to penicillin, tylosin, vancomycin, streptomycin, chloramphenicol, neomycin, colistin, and to S S Agar, Staphylococcus Medium No. 110, and Potato Dextrose Agar were recorded, together with Gram reaction and cell morphology. This information was then fed into an IBM 1410 digital computer which grouped and analyzed each isolate into 10 microbial genera, or groups, according to the identification key. The identification scheme was established by use of reference culture studies and from the literature. This system was used to analyze the microbial flora in dover sole (Microstomus pacificus) and ground beef. The method described in this article enables one to examine large numbers of microbial isolates with simplicity.

FOOTNOTES

2 Present address: Research Laboratories, California Packing Corp., 215 Fremont St., San Francisco, Calif.

1 Technical Paper no. 2007 from Oregon Agricultural Experiment Station, Corvallis.

Appl Environ Microbiol. 1965 September; 13(5): 808-817
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1965 by the American Society for Microbiology. All rights reserved.