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Appl Environ Microbiol. 1965 November; 13(6): 895-898
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Level and Specificity of Antibodies Evoked by Crude and Purified Antigens of Poliovirus I and Echovirus 7

Laurence H. Frommhagen

Exobiology Division, National Aeronautics and Space Administration, Ames Research Center, Moffet Field, California

ABSTRACT

Preparations of poliovirus I and echovirus 7, purified by density gradient centrifugation, liquid-phase partition, and anion exchange (diethylaminoethyl) chromatography, have been shown to evoke high antibody levels of substantial specificity in the complement-fixation assay. Certain practical aspects of the three purification methods are discussed. These results argue for the use of purified viral antigens, particularly in view of the simplicity of the purification methods now available.


Appl Environ Microbiol. 1965 November; 13(6): 895-898
Copyright © 1965 American Society for Microbiology. All Rights Reserved.







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Copyright © 1965 by the American Society for Microbiology. All rights reserved.