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Appl Environ Microbiol. 1966 January; 14(1): 12-20
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Enrichment Medium for Selection of Salmonella from Fish Homogenate¹

Department of Microbiology, California State College, Long Beach, California

ABSTRACT

A new liquid medium, called "dulcitol selenite enrichment," has been developed for the detection and enumeration of Salmonella in foods. The medium is not only highly selective in enriching Salmonella and inhibiting completely or appreciably other extraneous organisms commonly found in seafoods, but is also highly sensitive in recovering as low as 2 to 7 cells of Salmonella, even in the presence of large numbers (10⁴ to 10⁶ cells) of mixed flora common to these foods. The addition of seafood material does not seem to interfere with the sensitivity, selectivity, or productivity of the medium. Even physiologically debilitated cells of Salmonella were enriched well enough in this medium to be detected easily.

¹ Part of this research was carried out at the College of Fisheries, University of Washington, Seattle, and was supported by Public Health Service research grant RG-5932 from the National Institutes of Health.