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Appl Environ Microbiol. 1966 January; 14(1): 60-69
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Enzymatic Techniques for the Study of Pathways of Carbohydrate Utilization in the Rumen¹

Department of Animal Husbandry, University of California, Davis, California

ABSTRACT

Enzymatic techniques were used to study the metabolism of carbohydrates by ruminal bacteria. A direct relationship was observed between the proportions of acetate and propionate formed and the specific activities of the enzymes which participate in forming these acids. An inverse relationship between butyrate formation and butyrate-forming enzymes was observed. The relative activities of succinic dehydrogenase to fumaric reductase, nicotinamide adenine dinucleotide-linked glutamic dehydrogenase to nicotinamide adenine dinucleotide phosphate-linked glutamic dehydrogenase, and pyridine nucleotide-nonlinked lactic dehydrogenase to pyridine nucleotide-linked lactic dehydrogenase were affected by the level of concentrates in the diet. Lactyl coenzyme A dehydrase activity was below the limits of the assay technique in many samples from the alfalfa hay diet, and increased to relatively high levels when concentrates were fed. It is suggested that the enzymatic method will prove valuable for studying the contributions of individual microorganisms to the overall ruminal metabolism, and, with certain limitations, useful for estimating the relative contributions of alternate pathways.

² Present address: Department of Dairy Science, University of Illinois, Urbana.

¹ Based on material taken from a dissertation submitted by D. L. Palmquist in partial fulfillment of the requirements for the Ph.D. degree, University of California, Davis. Presented in part at the 49th Annual Meeting of the Federated American Societies for Experimental Biology, Atlantic City, N.J., April 1965.