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Appl Environ Microbiol. 1966 March; 14(2): 178-182
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

ß-Galactosidase for Distinguishing between Citrobacter and Salmonella

Department of Bacteriology, University of California, Los Angeles, California

ABSTRACT

Detection of ß-galactosidase with the aid of o-nitrophenyl-ß-D-galactopyranoside (ONPG) was examined as a means for distinguishing between Citrobacter and Salmonella. Several factors which influence sensitivity and reliability of the test were studied. A bacteriostat, sodium azide, was included to permit prolonged incubation of weak and negative strains of enteric bacilli. By the procedure described, salmonellae gave negative ONPG tests; all of 171 strains of Citrobacter gave positive tests.