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Appl Environ Microbiol. 1966 March; 14(2): 212-217
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Biochemical Differentiation of the Enterobacteriaceae with the Aid of Lysine-Iron-Agar

Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts
Department of Bacteriology, Massachusetts General Hospital, Boston, Massachusetts
Communicable Disease Center, U.S. Public Health Service, Atlanta, Georgia

ABSTRACT

A procedure is described for identifying members of the family Enterobacteriaceae isolated from clinical specimens. The methods are based on primary differentiation of the various groups of bacteria by the use of Kligler Iron Agar and lysine-iron-agar. For identification of Salmonella, Shigella, and Arizona group organisms from stools, Triple Sugar Iron Agar and lysine-iron-agar are employed. The usefulness of this schema for diagnostic bacteriology laboratories is discussed. It is not intended to replace methods used in reference or research laboratories.