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Appl Environ Microbiol. 1966 March; 14(2): 267-270
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Modified Agglutination Test for Pasteurella tularensis

Naval Medical Research Unit #1, and Naval Biological Laboratory, School of Public Health, University of California, Berkeley, Oakland, California

ABSTRACT

Several modifications in technique were incorporated into the standard agglutination test for Pasteurella tularensis. Reciprocal shaking of all tubes in a Kahn shaker was introduced to increase the rate of agglutination and quantity of agglutinated cell mass, making it possible to report preliminary results within 4 hr. Increased incubation time at a higher temperature was used to favor the rate of agglutination. A serum control for each serum tested was necessary to detect false positive tests. Finally, a verification procedure with 5% NaCl used as the diluent was instituted to prevent these false positive reactions.