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Appl Environ Microbiol. 1966 March; 14(2): 280-283
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Influence of Cobalt on Fermentative Methylation

Research Division, Bristol Laboratories, Division of Bristol-Myers, Syracuse, New York

ABSTRACT

Streptomyces rishiriensis produces at least five closely related antibiotics. Strain selection yielded a culture producing only the most active component, coumermycin A. Hydrolysis of this antibiotic by barium hydroxide yielded both 5-methyl-pyrrole-2-carboxylic acid and pyrrole-2-carboxylic acid, which could be separated by paper chromatography. Coumermycin A was thus shown to be two fractions, designated A₁ and A₂ depending upon the nature of the pyrrole carboxylic acid portion. The addition of cobalt to the fermentation medium at a level as low as 0.01 µg/ml shifted the fermentation exclusively to the production of coumermycin A₁. Other ions were ineffective, except nickel, whose activity could be explained by the presence of contaminating cobalt.