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Appl Environ Microbiol. 1966 March; 14(2): 292-294
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Pilot Plant Glycerol Production with a Slow-Feed Osmophilic Yeast Fermentation

Department of Biochemistry, University of Wisconsin, Madison, Wisconsin
Forest Products Laboratory,¹ Forest Service, U.S. Department of Agriculture, Madison, Wisconsin

ABSTRACT

A slow feed batch fermentation is described for the production of glycerol from sugar. The conversion efficiency was approximately 1 mole of glycerol produced per mole of glucose utilized after the cell growth phase. The glycerol production phase was extended several-fold by periodic glucose addition. The yeast cell count remained constant during this time as limited by phosphate, a deficiency required for an efficient glycerol fermentation. A small amount of phosphate was supplied during the extended fermentation, maintaining an active culture, by the normal autolysis of spent cells. Interfering or inhibitory by-products did not accumulate, and the osmophilic yeasts are tolerant of high glycerol concentrations. These factors combined to allow a particularly efficient fermentation well suited to product enrichment by supplying large quantities of substrate over an extended period.

¹ Maintained in cooperation with the University of Wisconsin.