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Appl Environ Microbiol. 1966 May; 14(3): 391-393
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Use of Bisulfite in the Streptococcal Anti-Nicotinamide Adenine Dinucleotidase Test

Department of Pediatrics, University of Minnesota School of Medicine, Minneapolis, Minnesota

ABSTRACT

A major drawback in the performance of the streptococcal anti-nicotinamide adenine dinucleotidase test on a large scale has been the hazard involved in the use of cyanide in assaying nicotinamide adenine dinucleotide. The use of bisulfite instead of cyanide in the performance of this test was investigated. The assays obtained with bisulfite parallel closely those obtained with cyanide, and justify the replacement of cyanide by the safer bisulfite reagent.

¹ Established Investigator, Helen Hay Whitney Foundation.