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Appl Environ Microbiol. 1966 November; 14(6): 839-844
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Potency of Adenovirus Vaccines: Comparison of Unconcentrated, Concentrated, and Soluble Antigen Preparations

The National Drug Company Research Laboratories, Philadelphia, Pennsylvania

ABSTRACT

Five adenoviruses were effectively concentrated and partially purified by methanol precipitation. The soluble antigens from one adenovirus concentrate were obtained by adsorption to and elution from calcium phosphate. Formaldehyde-inactivated vaccines prepared from the virus concentrates were more antigenic in guinea pigs than a National Institutes of Health reference vaccine or one prepared from unconcentrated adenovirus. The soluble antigens also proved to be antigenic. The data indicate that adenovirus vaccines of superior potency can be prepared from concentrated virus preparations and that the extracted soluble antigens are immunogenic.

¹ Present address: Division of Research Facilities and Resources, National Institutes of Health, Bethesda, Md.

² Present address: Northeast Shellfish Sanitation Research Center, U.S. Public Health Service, Narragansett, R.I.