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Appl Environ Microbiol. 1966 November; 14(6): 899-901
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Adaptation of Pseudomonas fluorescens to Low Levels of Water Activity Produced by Different Solutes¹

Department of Bacteriology, The University of Wisconsin, Madison, Wisconsin

ABSTRACT

A culture of Pseudomonas fluorescens which grew at a minimal a_w of 0.97 in a glucose-salts broth adjusted to that level with NaCl, KCl, or glucose, and at a_w 0.9725 with Na₂SO₄, was adapted to grow at lower a_w levels produced by these solutes. Adaptation to growth at a low a_w produced by means of one solute did not necessarily result in growth at that a_w when another solute was employed to lower the a_w. The culture adapted to grow at a_w 0.9650 produced by addition of glucose could grow equally well in broth adjusted to that a_w with NaCl or KCl, but not Na₂SO₄. NaCl and KCl permitted growth at the lowest a_w level (0.9625), and the culture adapted in KCl did equally well in NaCl.

¹ Published with the approval of the Director of the Wisconsin Agricultural Experiment Station.