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Appl Environ Microbiol. 1966 November; 14(6): 985-988
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Safe Self-contained Carbon Dioxide-Hydrogen Anaerobic System

Becton, Dickinson and Co. and Baltimore Biological Laboratory, Baltimore, Maryland

ABSTRACT

An anaerobic system is described which allows the microbiologist or hospital technician ease of operation not previously possible with other systems. Gas cylinders, vacuum pumps, valves, and gauges have been eliminated. A new anaerobic lid was developed that is fitted only with a snap-in rubber gasket, a double stainless-steel gauze flash arrestor, and a catalyst holder. The holder contains a palladium pellet catalyst which is active at room temperature and requires no heat for activation. This system was made specifically for use with a disposable hydrogen-carbon dioxide generator and a disposable methylene blue anaerobic indicator. In addition to ease of operation, this jar incorporates unique safety features which eliminate the possibility of a laboratory explosion. An oxygen-free atmosphere composed primarily of nitrogen and carbon dioxide was quickly achieved within the jar to insure maximal growth.