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Appl Environ Microbiol. 1967 January; 15(1): 117-119
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Isolation of Thermophiles from Broadleaf Tobacco and Effect of Pure Culture Inoculation on Cigar Aroma and Mildness

Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio

ABSTRACT

Thermophilic members of the genus Bacillus isolated from fermented Connecticut broadleaf tobacco included eight strains of B. subtilis, five strains of B. coagulans, four strains of B. megaterium, and three strains of B. circulans. Some of these strains in pure single or mixed culture were employed to enrich the normal thermophile flora of "sweating" tobacco. Three strains of B. subtilis and one of B. circulans, either in single or multiple enrichment, caused the more rapid appearance of a pleasing aroma in Pennsylvania "Wrapper B" filler tobacco. These conclusions are based on subjective reactions of professional testers after numerous blindfold smoking tests.

¹ Present address: John Berger and Sons Tobacco Co., Cincinnati, Ohio.