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Appl Environ Microbiol. 1967 January; 15(1): 163-165
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Viability of Bacillus popilliae after Lyophilization of Liquid Nitrogen Frozen Cells¹

Department of Bacteriology, Kansas State University, Manhattan, Kansas

ABSTRACT

The per cent viability of Bacillus popilliae after lyophilization of liquid nitrogen frozen cells was determined. Lyophilization of 9- to 12-hr cells which had been suspended in 5% sodium glutamate plus 0.5% gum tragacanth, frozen in liquid nitrogen vapor, and dried 4 to 5 hr with the ampoules exposed to room temperature resulted in survival of 64.6% of the original cells. After storage of these lyophilized preparations for 6 months at room temperature, 10.5% of the original cells were still viable.

¹ Contribution no. 437, Department of Bacteriology, Kansas Agricultural Experiment Station, Manhattan. Portion of a thesis submitted by the the senior author in partial fulfillment of the requirements for the M.S. degree in bacteriology at Kansas State University.