This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Krabbenhoft, K. L. Articles by Elliker, P. R. Search for Related Content PubMed Articles by Krabbenhoft, K. L. Articles by Elliker, P. R. Agricola Articles by Krabbenhoft, K. L. Articles by Elliker, P. R.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1967 January; 15(1): 178-185
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Influence of Culture Media on the Radiation Resistance of Micrococcus radiodurans

Department of Microbiology, Oregon State University, Corvallis, Oregon

ABSTRACT

The addition of NZ-case (a tryptic digest of casein) to a growth medium (PC) consisting of tryptone, glucose, and yeast extract caused a significant decrease in radiation resistance of Micrococcus radiodurans. The level of radiation resistance was inversely related to the concentration of NZ-case. The LD₅₀ for this organism was approximately 700 krad when grown in tryptone, glucose, yeast extract, and DL-methionine (TGYM) broth, but it was approximately one-half as resistant when grown in a PC medium containing 0.5% NZ-case (PCNZ). The resistance to ultraviolet light was also reduced. Cultures transferred from PCNZ to TGYM media regained the high level of resistance.

¹ Present address: Department of Biology, New Mexico State University, Las Cruces.