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Appl Environ Microbiol. 1967 January; 15(1): 186-190
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer

Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois

ABSTRACT

A method has been developed for the production of aflatoxin by growing Aspergillus flavus NRRL 3145 on solid substrate wheat. Optimal yields of 900 µg of aflatoxin G₁ and 900 µg of aflatoxin B₁ per g of substrate were obtained in 4 to 5 days at 28 C. A study of aflatoxin production on hulls and groats of oats and on whole oats by A. flavus strains NRRL 2999, NRRL 3000, and NRRL 3145 revealed that aflatoxin was produced on all three substrates, although production was very slight on hulls. Strain NRRL 3145 grown on solid substrate groats produced the largest amounts of aflatoxin: 580 µg of B₁ and 450 µg of G₁ per g of substrate. A densitometric method for reading thin-layer chromatographic plates is described; this is more objective and more accurate than the visual methods previously used for the determination of all four aflatoxins.