This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Whitman, J. E. Articles by Hetrick, F. M. Search for Related Content PubMed Articles by Whitman, J. E. Articles by Hetrick, F. M. Agricola Articles by Whitman, J. E. Articles by Hetrick, F. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1967 January; 15(1): 62-66
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Purification of the Kilham Rat Virus

Department of Microbiology, University of Maryland, College Park, Maryland

ABSTRACT

A purification procedure is described for the isolation of Kilham rat virus (RV) from infected suckling hamster kidney and liver suspensions. The procedure involved a combination of sonic treatment, differential centrifugation, butanol-chloroform extraction, agar column flow diffusion, and potassium tartrate density gradient centrifugation. The purified virus retained its infectivity and was specifically neutralized by RV hyperimmune antiserum. Electron micrographs from the RV band (density 1.31 g/ml) showed numerous homogeneous particles approximately 22 mµ in diameter.

¹ Present address: Division of Virus and Cell Biology Research, Merck Institute for Therapeutic Research, West Point, Pa.