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Appl Environ Microbiol. 1967 March; 15(2): 219-223
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Dense Cultures of Neisseria gonorrhoeae in Liquid Medium

R. Brookes and C.-G. Hedén

Department of Bacteriology, Karolinska Institutet, Stockholm, Sweden

ABSTRACT

Cultivation of Neisseria gonorrhoeae was effected in a conical glass culture vessel surrounded by a constant-temperature water jacket, and with facilities for stirring, aeration, and pH measurement and control. With the use of an aerated peptone-based medium, containing polypropylene glycol to prevent foam build-up, the yields obtained over the pH range from 5.8 to 7.4 were determined. The greatest yield was obtained at pH 6.4 when the dry weight was 1.5 g/liter. At pH 7.2 to 7.6, lysis was extensive.

Appl Environ Microbiol. 1967 March; 15(2): 219-223
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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Copyright © 1967 by the American Society for Microbiology. All rights reserved.