This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wagner, R. B. Articles by Ruchman, I. Search for Related Content PubMed Articles by Wagner, R. B. Articles by Ruchman, I. Agricola Articles by Wagner, R. B. Articles by Ruchman, I.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1967 March; 15(2): 239-242
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Method for Determining Antineuraminidase Activity

The Wm. S. Merrell Company, Cincinnati, Ohio

ABSTRACT

A test was developed to screen drugs for antineuraminidase (influenza sialidase) activity in vitro. Neuraminidase prepared from Vibrio cholerae was added to a substrate containing ganglioside, prepared from calf brain. Sialic acid is a split product in the reaction. The presence of sialic acid was detected colorimetrically by use of Warren's Thiobarbituric Acid Assay after drugs had been added to inhibit the action of neuraminidase on the calf brain substrate.

¹ Present address: Sterling-Winthrop Research Institute, Rensselaer, N.Y.

² Present address: Department of Microbiology, University of Kentucky, Lexington.