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Appl Environ Microbiol. 1967 March; 15(2): 243-244
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Modified Procedure for the Microscopic and Macroscopic Study of Viral Plaques

John A. Hartford Research Laboratory, Madison General Hospital, Madison, Wisconsin
Department of Pediatrics, University of Wisconsin School of Medicine, Madison, Wisconsin

ABSTRACT

A virus plaque method that consistently gives good visual contrast for macroscopic observation and also permits microscopic study is described. Cells are grown in plastic flasks and the gelled overlay medium can be of any desired agar concentration or volume. A fixing solution is used prior to removal of the agar overlay, and dye is added to the fixing solution or staining can follow fixation and agar removal. The bottom of the flask with the fixed monolayer is separated from the rest of the container and handled as a slide. A new mounting medium is described.